embedding, sectioning and h&e staining  (Excalibur Pathology Inc)

Journal: Nature Communications

Article Title: Endocrine-exocrine miR-503-322 drives aging-associated pancreatitis via targeting MKNK1 in acinar cells

doi: 10.1038/s41467-025-57615-x

Figure Lengend Snippet: A qPCR analysis for pancreatic Pri-miR-503 expression in 8-week-old control (WT), PKI heterozygous (PKI/WT) and PKI homozygous (PKI/KI) male mice. n = 5. B Weight monitoring in WT, PKI/WT and PKI/KI male mice. n = 4. C , D Photograph of the pancreas ( C ), representative sections of of H&E, Masson, F4/80 and CK19 immunofluorescence staining in pancreas of 8-week-old PKI male mice ( D ). n = 3 mice. E Pancreatic weights after calibration with body weight in 8-week-old PKI male mice. WT, n = 6; PKI/WT, n = 5; PKI/KI, n = 3. F Survival curves for WT, PKI/WT and PKI/KI male mice. G Schematic of acinar cell-specific miR-503-322 knock-in (EKI) mice: 6–8 weeks WT and EKI male or female mice were injected intraperitoneally ( ip .) with tamoxifen solution, 100 mg/kg, in corn oil, for three consecutive days and sacrificed 3 days after the last tamoxifen injection. H qPCR analysis of Pri-miR-503 in acinar cells of WT and EKI male mice after three times tamoxifen-induced. n = 4. I Representative sections of pancreas of H&E, receptor-interacting serine-threonine kinase 3 (RIPK3) immunohistochemistry and immunofluorescence staining of F4/80 after first tamoxifen injection 5 days in WT and EKI male mice. Arrows indicate the macrophages. n = 4 mice. J Pancreatic histological scores, quantitation of average optical density of RIPK3 and the number of F4/80 positive cells in pancreatic sections under ×600 microscopic view for I . n = 5 mice, and at least 10 photographs were taken for statistical analysis. K Survival curves for WT and EKI male and female mice. L Pancreatic H&E of WT and EKI female mice after first tamoxifen injection 28 days. M Representative sections of pancreatic Masson dyeing from EKI male mice after first tamoxifen injection 28 days. ADM, Acinar-to-ductal metaplasia. n = 4 mice. Data are means ± SEM. Data were analyzed using one-way ANOVA ( A , E ), two-way ANOVA with Tukey test ( B ), unpaired Student’s t tests ( H , J ) or Survival cure analyses ( F , K ). Source data are provided as a Source Data file.

Article Snippet: Paraffin embedding, serial sectioning, H&E and Masson staining of all samples were commissioned from Servicebio Technologies.

Techniques: Expressing, Control, Immunofluorescence, Staining, Knock-In, Injection, Immunohistochemistry, Quantitation Assay

Journal: Nature Communications

Article Title: Endocrine-exocrine miR-503-322 drives aging-associated pancreatitis via targeting MKNK1 in acinar cells

doi: 10.1038/s41467-025-57615-x

Figure Lengend Snippet: A Representative images of H&E and Masson staining of pancreatic sections from the young adult (YA) and the elderly adult (EA); quantitation of collagen volume fraction. The dashed area indicates acini. n = 10 and a total of 30 photographs were taken for statistical analysis. B Representative images of immunofluorescence staining of PCNA (green) in pancreatic sections and counted the number of PCNA-positive cells. n = 10. Arrows indicate proliferating acinar cells. n = 10 and a total of 30 photographs were taken for statistical analysis. C In situ hybridization of miR-503 (40 nM) in young and elderly pancreatic sections. Scramble-RNA was negative reference (40 nM), and U6 was positive reference (0.1 nM). The dotted line indicates islet, and the solid line is exocrine. n = 3 independent experiments. D Representative images of immunofluorescence staining of MKNK1 (green) and amylase (red) in pancreatic sections from young and elderly people and quantitation of amylase and MKNK1 mean fluorescence intensity. n = 10. E Serum amylase assay of the young adult (YA), the elderly adult (EA) and the elderly adult with diabetes (EA + DM). YA group, n = 65. EA group, n = 65. EA + DM, n = 30. F MiR-503 concentration in human serum of YA, EA and EA + DM. YA group, n = 45. EA group, n = 45. EA + DM, n = 30. G Correlation analysis of amylase levels of human serum and age. Each point represents one people ( n = 160). Correlation coefficient (R) and p value from simple linear regression are shown. H Correlation analysis of miR-503 concentration in human serum and serum amylase levels. Each point represents one people ( n = 120). Correlation coefficient (R) and P value from simple linear regression are shown. Box plots with centerline = median, box = 25th–75th percentile, and whiskers = 5th–95th percentile, outliers = open circles ( A , B , D – F ). Data are means ± SEM. Data were analyzed using unpaired Student’s t tests ( A , B , D ), one-way ANOVA with Tukey test ( E , F ) or Correlation analysis ( G , H ). Source data are provided as a Source Data file.

Article Snippet: Paraffin embedding, serial sectioning, H&E and Masson staining of all samples were commissioned from Servicebio Technologies.

Techniques: Staining, Quantitation Assay, Immunofluorescence, In Situ Hybridization, Fluorescence, Concentration Assay